Whole Genome Sequence Quality Control and Validation

Do you regularly sequence microbial genomes and is it important for you that the results can be trusted? Do you need to establish proficiency testing for a sequencing laboratory or competency testing for lab staff?

If you can answer yes to one of the above questions, please read on.

GoSeqIt has developed a system for quality control and validation of bacterial whole genome sequence data, which we offer to anyone for whom it is of essence to be able to trust the data produced by their sequencing machine. The validation panel currently comprises six organisms:

IDSpeciesATCC strain IDGC content (%)Size (bp)Division
C2Aeromonas hydrophilaATCC 7966624744448gram-negative
C3Escherichia coliATCC 8739514746218gram-negative
C5Staphylococcus aureusATCC 25923332778854gram-positive
C6Salmonella enterica subsp. enterica serovar TyphimuriumATCC 14028524870265gram-negative
C46Enterococcus faecalisATCC 29212382939973gram-positive
C55Escherichia coliATCC 25922515130767gram-negative

How does it work?

  1. You order the isolates you wish to have included in the test via ATCC or a similar culture collection.
  2. You extract DNA using the procedure you normally use and perform whole genome sequencing on your Illumina sequencer. 
  3. You share the output from the sequencing run with GoSeqIt via the Illumina Basespace Sequence Hub.
  4. Within three working days following the sharing of data, you receive a comprehensive report providing a complete picture of the quality of your sequence data. If any of the quality parameters do not meet the expected thresholds, the report will additionally contain advice on how to troubleshoot procedures, consumables, and equipment to ensure optimal quality of your sequence data.

More Information:

Download whitepaper on GoSeqIt's Whole Genome Sequence Quality Control and Validation Scheme: Download PDF

If you would like us to contact you, please provide name, email, and any questions in the form below.

You can also contact us directly by mail: mail@goseqit.com

We look forward to ensure the quality of your sequence data, so you can focus on using it for making great discoveries!